Journal article
Induction and antiviral activity of ferret myxovirus resistance (Mx) protein 1 against influenza A viruses
R Farrukee, LSU Schwab, JB Barnes, AG Brooks, SL Londrigan, G Hartmann, T Zillinger, PC Reading
Scientific Reports | NATURE PORTFOLIO | Published : 2024
Abstract
Myxovirus resistance (Mx) proteins are products of interferon stimulated genes (ISGs) and Mx proteins of different species have been reported to mediate antiviral activity against a number of viruses, including influenza A viruses (IAV). Ferrets are widely considered to represent the ‘gold standard’ small animal model for studying pathogenesis and immunity to human IAV infections, however little is known regarding the antiviral activity of ferret Mx proteins. Herein, we report induction of ferret (f)Mx1/2 in a ferret lung cell line and in airway tissues from IAV-infected ferrets, noting that fMx1 was induced to higher levels that fMx2 both in vitro and in vivo. Overexpression confirmed cytop..
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Awarded by Department of Health and Aged Care, Australian Government
Funding Acknowledgements
This work was supported by Project Grant APP1143154 from the National Health and Medical Research Council (NHMRC) of Australia; the German Center for Infectious Diseases (grant TTU 07.834_00 to G. H); and the Deutsche Forschungsgemeinschaft (German Research Foundation) (project 369799452-TRR237 to G. H. and T.Z, IRTG2168 to G.H., and under Germany's Excellence Strategy-EXC2151- 390873048, of which G. H. is a member). This project was supported by a fellowship grant from the Global Virus Network (to R.F.). The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health. We thank the Melbourne Flow Cytometry Core Platform for assistance with flow cytometric analysis.